本文關(guān)鍵詞：添加羧化殼聚糖采血后即刻分離制備血小板可行性研究　出處：《中國(guó)輸血雜志》2017年07期 　論文類型：期刊論文

  更多相關(guān)文章： 血液成分制備 血小板制備 殼聚糖 甲殼素

【摘要】：目的探討使用甲殼素衍生物—羧化殼聚糖即刻分離制備血小板的可行性,尋找血液成分分離新方向。方法隨機(jī)選取大連地區(qū)獻(xiàn)血者40名,當(dāng)日成功獻(xiàn)血時(shí)同步采集全血10 m L,分成實(shí)驗(yàn)組,自然沉降對(duì)照組和離心對(duì)照組進(jìn)行實(shí)驗(yàn)。從阿拉丁試劑公司購(gòu)買水溶性殼聚糖,用血液保存液Ⅱ配制成(0.25~2.0)mg/m L不同濃度溶液,按1∶4比例加入到2 m L全血中,靜置4 h,觀察血液分離速度,計(jì)數(shù)血漿中紅細(xì)胞,白細(xì)胞及血小板,觀察血小板聚集現(xiàn)象及紅細(xì)胞形態(tài)變化;在最適殼聚糖添加濃度的紅細(xì)胞中加入適量紅細(xì)胞保存液予以4℃保存,35 d保存期內(nèi)檢測(cè)溶血率。結(jié)果加入1.0~2.0 mg/m L濃度殼聚糖的實(shí)驗(yàn)組血液分離速度均明顯快于自然沉降對(duì)照組,血漿中均僅存微量紅細(xì)胞,富血小板,35d時(shí)無(wú)明顯溶血,同離心對(duì)照組。結(jié)論殼聚糖可以用于采血后即刻分離富血小板血漿。
[Abstract]:Objective to investigate the feasibility of using chitin derivative carboxylated chitosan to prepare platelets and to find a new direction of blood component separation. Methods 40 blood donors from Dalian were randomly selected. During the successful blood donation on the same day, the whole blood was collected at the same time and divided into experimental group, natural sedimentation control group and centrifugal control group. The water-soluble chitosan was purchased from Aladdin reagent company. Blood preservation solution 鈪，

本文編號(hào)：1403160